Secretory inhibitor of lysozyme and biofilm formation of intestinal strains Candida in children with reactive arthritis
https://doi.org/10.31146/2949-4664-apps-2-2-5-11
Abstract
This short communications compares the phenotypic properties of Candida species isolated from gut of children with reactive arthritis, such as secretory inhibitor of lysozyme and biofilm formation. A total of 65 clinical strains of Candida species isolated from the feces of healthy children and children with ReA were tested. SIL production of Candida by inhibiting lysozyme activity against Micrococcus luteus ATCC 15307 were studied (mg/mL*OD). Quantitative assessment of biofilm formation was performed using crystal violet binding assay method (CU). A significantly higher proportion of ReA strains were SIL-positive (77.8% vs. 40.0%) and BF-positive (64.4% vs. 30.0%) compared with non-ReA strains (P < 0.01). These results suggest that the functional significance of SIL production and biofilm formation must be estimated, of investigating the roles of intestinal of Candida in the pathogenesis of spondylarthritis, including reactive arthritis. It is imperative to delineate microbial factors that contribute to ReA development.
About the Authors
O. V. Bukharin
Institute of Cellular and Intracellular Symbiosis, Ural department, Russian Academy of Science
Russian Federation
Russian Federation
N. B. Perunova
Institute of Cellular and Intracellular Symbiosis, Ural department, Russian Academy of Science
Russian Federation
Russian Federation
E. V. Ivanova
Institute of Cellular and Intracellular Symbiosis, Ural department, Russian Academy of Science
Russian Federation
Russian Federation
O. E. Chelpachenko
Institute of Cellular and Intracellular Symbiosis, Ural department, Russian Academy of Science
Russian Federation
Russian Federation
A. I. Khavkin
Research Clinical Institute of Childhood, Ministry of Health care of the Moscow Region; Federal State Autonomous Educational Institution of Higher Education “N. I. Pirogov Russian National Research Medical University” of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
References
1. Stavropoulos P.G., Soura E., Kanelleas A., Katsambas A., Antoniou C. Reactive arthritis. J Eur Acad Dermatol Venereol. 2015 Mar;29(3):415-24. doi: 10.1111/jdv.12741.
2. García-Kutzbach A., Chacón-Súchite J., García-Ferrer H., Iraheta I. Reactive arthritis: update 2018. Clin Rheumatol. 2018 Apr;37(4):869-874. doi: 10.1007/s10067-018-4022-5.
3. Yeoh N., Burton J. P., Suppiah P., Reid G., Stebbings S. The role of the microbiome in rheumatic diseases. Curr Rheumatol Rep. 2013 Mar;15(3):314. doi: 10.1007/s11926-012-0314-y.
4. Sekirov I., Russell S. L., Antunes L. C., Finlay B. B. Gut microbiota in health and disease. Physiol Rev. 2010 Jul;90(3):859-904. doi: 10.1152/physrev.00045.2009.
5. Bukharin O.V., Perunova N. B. Microsymbiocenosis. Ekaterinburg. UrDepart RAS. 2014. (in Russ.)
6. Sonoyama K., Miki A., Sugita R., Goto H., Nakata M., Yamaguchi N. Gut colonization by Candida albicans aggravates inflammation in the gut and extra-gut tissues in mice. Med Mycol. 2011 Apr;49(3):237-47. doi: 10.3109/13693786.2010.511284.
7. Chelpachenko OE, Bukharin OV, Danilova EI, Fedotova LP, Perunova NB, et al. A clinical case of Candida arthritis in a child. Vopr. prakt. pediatr. (Clinical Practice in Pediatrics). 2017;(3):77-82. (in Russ.) doi:10.20953/1817-7646-2017-3-77-82.
8. Höfs S., Mogavero S., Hube B.Interaction of Candida albicans with host cells: virulence factors, host defense, escape strategies, and the microbiota. J Microbiol. 2016 Mar;54(3):149-69. doi: 10.1007/s12275-016-5514-0.
9. Borghi E., Romagnoli S., Fuchs B. B., Cirasola D., Perdoni F., Tosi D., Braidotti P., Bulfamante G., Morace G., Mylonakis E. Correlation between Candida albicans biofilm formation and invasion of the invertebrate host Galleria mellonella. Future Microbiol. 2014;9(2):163-73. doi: 10.2217/fmb.13.159.
10. Deckers D., Vanlint D., Callewaert L., Aertsen A., Michiels C. W. Role of the lysozyme inhibitor Ivy in growth or survival of Escherichia coli and Pseudomonas aeruginosa bacteria in hen egg white and in human saliva and breast milk. Appl Environ Microbiol. 2008 Jul;74(14):4434-9. doi: 10.1128/AEM.00589-08.
11. Bukharin O. V. Persistence of pathogenic bacteria. Moscow. Medicine Publ., 1999. (in Russ.)
12. Ivanov I.B., Gritsenko V. A., Kuzmin M. D. Phenotypic differences between coryneform bacteria isolated from seminal fluid of healthy men and men with chronic prostatitis syndrome. Asian J Androl. 2009 Jul;11(4):517-20. doi: 10.1038/aja.2009.24.
13. O’Toole G.A., Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998 May;28(3):449-61. doi: 10.1046/j.1365-2958.1998.00797.x.
14. da Silva Dantas A., Lee K. K., Raziunaite I., Schaefer K., Wagener J., Yadav B., Gow N. A. Cell biology of Candida albicans-host interactions. Curr Opin Microbiol. 2016 Dec;34:111-118. doi: 10.1016/j.mib.2016.08.006.
Review
For citations:
Bukharin O.V., Perunova N.B., Ivanova E.V., Chelpachenko O.E., Khavkin A.I. Secretory inhibitor of lysozyme and biofilm formation of intestinal strains Candida in children with reactive arthritis. Archives of Pediatrics and Pediatric Surgery. 2023;1(2):5-11. (In Russ.) https://doi.org/10.31146/2949-4664-apps-2-2-5-11
Views:
111
Email this article 